Correction of Microplate Data from High-Throughput Screening

  • Yuhong WangEmail author
  • Ruili Huang
Part of the Methods in Molecular Biology book series (MIMB, volume 1473)


High-throughput screening (HTS) makes it possible to collect cellular response data from a large number of cell lines and small molecules in a timely and cost-effective manner. The errors and noises in the microplate-formatted data from HTS have unique characteristics, and they can be generally grouped into three categories: run-wise (temporal, multiple plates), plate-wise (background pattern, single plate), and well-wise (single well). In this chapter, we describe a systematic solution for identifying and correcting such errors and noises, mainly basing on pattern recognition and digital signal processing technologies.

Key words

HTS Data correction Concentration response In vitro assay 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.National Center for Advancing Translational SciencesNational Institutes of HealthBethesdaUSA

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