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Efficient Assembly of DNA Using Yeast Homologous Recombination (YHR)

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Synthetic DNA

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1472))

Abstract

The assembly of multiple DNA parts into a larger DNA construct is a requirement in most synthetic biology laboratories. Here we describe a method for the efficient, high-throughput, assembly of DNA utilizing the yeast homologous recombination (YHR). The YHR method utilizes overlapping DNA parts that are assembled together by Saccharomyces cerevisiae via homologous recombination between designed overlapping regions. Using this method, we have successfully assembled up to 12 DNA parts in a single reaction.

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References

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Acknowledgements

This work was funded by Defense Advanced Research Projects Agency (DARPA) Living Foundries grant HR001-12-3-0006.

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Authors and Affiliations

  1. Amyris, Inc., 5885 Hollis Street, Suite 100, Emeryville, CA, 94608, USA

    Sunil Chandran & Elaine Shapland

Authors
  1. Sunil Chandran
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  2. Elaine Shapland
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Corresponding author

Correspondence to Sunil Chandran .

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Editors and Affiliations

  1. Applied Research Laboratories, The University of Texas at Austin, AUSTIN, Texas, USA

    Randall A. Hughes

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© 2017 Springer Science+Business Media New York

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Chandran, S., Shapland, E. (2017). Efficient Assembly of DNA Using Yeast Homologous Recombination (YHR). In: Hughes, R. (eds) Synthetic DNA. Methods in Molecular Biology, vol 1472. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6343-0_14

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  • DOI: https://doi.org/10.1007/978-1-4939-6343-0_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6341-6

  • Online ISBN: 978-1-4939-6343-0

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