Abstract
The visualization of meiotic chromosomes and their associated protein structures in both wild-type and mutant cells adds valuable insight into the molecular pathways that underlie reproductive cell formation. Here we describe basic methodology for visualizing meiotic chromosomes in a long-standing model organism for investigating the molecular and cell biology of meiosis, the budding yeast, S. cerevisiae. This chapter furthermore highlights a variety of conditional expression regimes that can be used to understand the dynamics and/or developmental constraints of chromosomal protein structures; such dynamic aspects of the macromolecular structures that mediate meiotic chromosome biology are typically not obvious from standard protein visualization experiments.
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References
Page SL, Hawley RS (2004) The genetics and molecular biology of the synaptonemal complex. Annu Rev Cell Dev Biol 20:525
Zickler D, Kleckner N (1999) Meiotic chromosomes: integrating structure and function. Annu Rev Genet 33:603
MacQueen AJ, Unpublished data
Voelkel-Meiman K, Moustafa SS, Lefrancois P, Villeneuve AM, MacQueen AJ (2012) Full-length synaptonemal complex grows continuously during meiotic prophase in budding yeast. PLoS Genet 8:e1002993
Voelkel-Meiman K et al (2013) SUMO localizes to the central element of synaptonemal complex and is required for the full synapsis of meiotic chromosomes in budding yeast. PLoS Genet 9:e1003837
White EJ, Cowan C, Cande WZ, Kaback DB (2004) In vivo analysis of synaptonemal complex formation during yeast meiosis. Genetics 167:51
Benjamin KR, Zhang C, Shokat KM, Herskowitz I (2003) Control of landmark events in meiosis by the CDK Cdc28 and the meiosis-specific kinase Ime2. Genes Dev 17:1524
Jambhekar A, Amon A (2008) Control of meiosis by respiration. Curr Biol 18:969
Rockmill B (2009) Chromosome spreading and immunofluorescence methods in Saccharomyes cerevisiae. Methods Mol Biol 558:3
Longtine MS et al (1998) Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 14:953
Xu L, Ajimura M, Padmore R, Klein C, Kleckner N (1995) NDT80, a meiosis-specific gene required for exit from pachytene in Saccharomyces cerevisiae. Mol Cell Biol 15:6572
Wakeling AE, Dukes M, Bowler J (1991) A potent specific pure antiestrogen with clinical potential. Cancer Res 51:3867
Rockmill B, Roeder GS (1998) Telomere-mediated chromosome pairing during meiosis in budding yeast. Genes Dev 12:2574
Loidl J, Klein F, Engebrecht J (1998) Genetic and morphological approaches for the analysis of meiotic chromosomes in yeast. Methods Cell Biol 53:257
Hollingsworth NM, Goetsch L, Byers B (1990) The HOP1 gene encodes a meiosis-specific component of yeast chromosomes. Cell 61:73
Smith AV, Roeder GS (1997) The yeast Red1 protein localizes to the cores of meiotic chromosomes. J Cell Biol 136:957
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MacQueen, A.J., Rockmill, B. (2017). Analysis of Meiotic Chromosome-Associated Protein Dynamics Using Conditional Expression in Budding Yeast. In: Stuart, D. (eds) Meiosis. Methods in Molecular Biology, vol 1471. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6340-9_8
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DOI: https://doi.org/10.1007/978-1-4939-6340-9_8
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6340-9
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