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Ribosome Profiling for the Analysis of Translation During Yeast Meiosis

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Meiosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1471))

Abstract

Ribosome profiling provides a genome-wide view of translation with unprecedented resolution. Application of this approach to fission and budding yeast revealed widespread regulation of translational efficiency, translation of short open reading frames on unannotated transcripts, and frequent translation of open reading frames in 5′ leader sequences. We present here a detailed protocol for the application of ribosome profiling to meiotic fission yeast cells, although the approach should be easily adapted to budding yeast.

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Acknowledgement

This was supported by a Biotechnology and Biological Sciences Research Council (BBSRC) project grant to Juan Mata (BB/M021483/1). We thank Maria J. Amorim for help with sucrose gradient centrifugation.

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Authors and Affiliations

  1. Department of Biochemistry, University of Cambridge, Building O, Downing Site, Cambridge, CB2 1QW, UK

    Caia Duncan & Juan Mata

Authors
  1. Caia Duncan
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  2. Juan Mata
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Corresponding author

Correspondence to Juan Mata .

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Editors and Affiliations

  1. Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada

    David T. Stuart

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Duncan, C., Mata, J. (2017). Ribosome Profiling for the Analysis of Translation During Yeast Meiosis. In: Stuart, D. (eds) Meiosis. Methods in Molecular Biology, vol 1471. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6340-9_4

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  • DOI: https://doi.org/10.1007/978-1-4939-6340-9_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6338-6

  • Online ISBN: 978-1-4939-6340-9

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