Abstract
Short hairpin RNA (shRNA)-pooled screening is a valuable and cost-effective tool for assaying the contribution of individual genes to cell viability and proliferation on a genomic scale. Here we describe the key considerations for the design and execution of a pooled shRNA screen to identify determinants of radiosensitivity.
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Ceroni, A., Higgins, G.S., Ebner, D.V. (2016). In Vitro-Pooled shRNA Screening to Identify Determinants of Radiosensitivity. In: Azorsa, D., Arora, S. (eds) High-Throughput RNAi Screening. Methods in Molecular Biology, vol 1470. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6337-9_9
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DOI: https://doi.org/10.1007/978-1-4939-6337-9_9
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6335-5
Online ISBN: 978-1-4939-6337-9
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