Abstract
RNAi has now become a valuable research tool for cell-based high-throughput screening. However, traditional RNAi high-throughput methods are based on multi-well plates, relying on expensive instruments and complicated operations. In this chapter, we describe a method termed self-assembled cell microarray (SAMcell), which integrates micro-fabrication, reverse transfection, and RNAi technologies and allows for cell behavior investigations to be performed directly on the cell chip. This method has been successfully employed to perform large-scale functional screening assays to identify gene modulators of cell migration, cell proliferation, and cellular apoptosis.
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Acknowledgement
We thank Suzhou Genoarray Biotech Co. Ltd. (Suzhou, China) for contributing to industrialization of SAMcell technology. This work was supported by projects of NSFC (Grant No. 81030040), MOST (Grant No. 2008ZX09401-002, 2011CB809106), and NSFC (20733001, 30600142) and Coulter Foundation Seed Grant.
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Zhang, H., Li, J. (2016). Self-Assembled Cell Microarray (SAMcell) for High-Throughput RNAi Screening. In: Azorsa, D., Arora, S. (eds) High-Throughput RNAi Screening. Methods in Molecular Biology, vol 1470. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6337-9_8
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DOI: https://doi.org/10.1007/978-1-4939-6337-9_8
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Online ISBN: 978-1-4939-6337-9
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