Abstract
RNAi screening of mammalian cells is often performed using siRNAs and cationic lipids as transfection reagents. Efficiency of transfection depends on growth characteristics of the cells and the cationic lipid used. With a large selection of cationic lipids available, it can often be difficult to select the optimal lipid and lipid:siRNA (vol:wt) ratio. Here, we describe the process of optimizing siRNA transfection conditions for efficient reverse transfection of mammalian cells using specific positive and negative siRNA controls.
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Montoya, J.J., Azorsa, D.O. (2016). Optimization of Transfection Conditions for siRNA Screening. In: Azorsa, D., Arora, S. (eds) High-Throughput RNAi Screening. Methods in Molecular Biology, vol 1470. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6337-9_2
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DOI: https://doi.org/10.1007/978-1-4939-6337-9_2
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6335-5
Online ISBN: 978-1-4939-6337-9
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