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Data Analysis for High-Throughput RNAi Screening

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High-Throughput RNAi Screening

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1470))

Abstract

High-throughput RNA interference (HT-RNAi) screening is an effective technology to help identify important genes and pathways involved in a biological process. Analysis of high-throughput RNAi screening data is a critical part of this technology, and many analysis methods have been described. Here, we summarize the workflow and types of analyses commonly used in high-throughput RNAi screening.

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Acknowledgements

The authors would like to thank Daniel H. Wai for his valuable contribution in preparing the heat map.

Author information

Authors and Affiliations

  1. Institute of Molecular Medicine, Phoenix Children’s Hospital, Phoenix, AZ, USA

    David O. Azorsa Ph.D.

  2. Department of Child Health, University of Arizona College of Medicine - Phoenix, Phoenix, AZ, USA

    David O. Azorsa Ph.D. & Megan A. Turnidge

  3. School of Life Sciences, Arizona State University, Tempe, AZ, USA

    Megan A. Turnidge

  4. Constellation Pharmaceuticals, Cambridge, MA, USA

    Shilpi Arora

Authors
  1. David O. Azorsa Ph.D.
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  2. Megan A. Turnidge
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  3. Shilpi Arora
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Corresponding author

Correspondence to David O. Azorsa Ph.D. .

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Editors and Affiliations

  1. Institute of Molecular Medicine, Phoenix Children’s Hospital, Phoenix, Arizona, USA

    David O. Azorsa

  2. Constellation Pharmaceuticals, Cambridge, Massachusetts, USA

    Shilpi Arora

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Azorsa, D.O., Turnidge, M.A., Arora, S. (2016). Data Analysis for High-Throughput RNAi Screening. In: Azorsa, D., Arora, S. (eds) High-Throughput RNAi Screening. Methods in Molecular Biology, vol 1470. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6337-9_19

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  • DOI: https://doi.org/10.1007/978-1-4939-6337-9_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6335-5

  • Online ISBN: 978-1-4939-6337-9

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