Abstract
Genomic DNA is dynamically associated with protein factors and folded to form chromatin fibers. The 3-dimensional (3D) configuration of the chromatin will enable the distal genetic elements to come into close proximity, allowing transcriptional regulation. Noncoding RNA can mediate the 3D structure of chromatin. Chromatin Interaction Analysis by Paired-End Tag Sequencing (ChIA-PET) is a valuable and powerful technique in molecular biology which allows the study of unbiased, genome-wide de novo chromatin interactions with paired-end tags. Here, we describe the standard version of ChIA-PET and a Multiplex ChIA-PET version.
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Acknowledgments
This work was supported by the National Research Foundation (NRF) Fellowship Grant to Melissa Jane Fullwood. The NRF grant number is R-713-000-143-281. In addition, this work is supported by NRF Funding and Ministry of Education funding to the Cancer Science Institute under the Research Centre of Excellence framework, as well as Yale-NUS and NTU start-up funds. This research is supported by the RNA Biology Center at the Cancer Science Institute of Singapore, NUS, as part of the funding under the Singapore Ministry of Education’s Tier 3 grants.
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Choy, J., Fullwood, M.J. (2017). Deciphering Noncoding RNA and Chromatin Interactions: Multiplex Chromatin Interaction Analysis by Paired-End Tag Sequencing (mChIA-PET). In: Ørom, U. (eds) Enhancer RNAs. Methods in Molecular Biology, vol 1468. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-4035-6_7
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DOI: https://doi.org/10.1007/978-1-4939-4035-6_7
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Publisher Name: Humana Press, New York, NY
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