Quality Control of Therapeutic Monoclonal Antibodies at the Hospital After Their Compounding and Before Their Administration to Patients

  • Emmanuel Jaccoulet
  • Claire Smadja
  • Myriam Taverna
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1466)

Abstract

Monoclonal antibodies (mAbs) are widely used in cancer therapy and recently many new mAbs have gained EMA and FDA approvals for oncology indications. Here we describe a highly reproducible CZE method, relying on a cationic coating allowing separation and identification of a complex mixture of four compounded mAbs widely used in cancer therapy (cetuximab, rituximab, bevacizumab, and trastuzumab).

Key words

Monoclonal antibodies Capillary electrophoresis Cationic coating Polybrene mAb identification Quality control Hospital 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Emmanuel Jaccoulet
    • 1
    • 2
  • Claire Smadja
    • 1
  • Myriam Taverna
    • 3
  1. 1.Institut Galien Paris-Sud, UMR 8612, Proteins and Nanotechnology in Analytical Science, CNRSUniv Paris-Sud, Univ Paris-SaclayChâtenay-MalabryFrance
  2. 2.Hôpital Européen Georges PompidouParisFrance
  3. 3.Institut Galien Paris-Sud, UMR 8612, Proteins and Nanotechnology in Analytical Science (PNAS), CNRSUniv. Paris-Sud, Université Paris-SaclayChâtenay-MalabryFrance

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