Abstract
Coculture assays allow the investigation of the role of endothelial cell and mural cell interactions in small vessel development and function. Different setups for coculture can be used to assay questions of interest. We include here methods for direct coculture, indirect coculture, and coculture in a three-dimensional extracellular matrix scaffold for studies of either a simple and direct association between the two cell types, the exchange of soluble molecules, or the interaction within a biomimetic tissue microenvironment.
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Acknowledgements
This work was supported by NIH grants R00EY021624 (JA) and R01EY005318 (PDA).
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Sánchez-Palencia, D.M., Bigger-Allen, A., Saint-Geniez, M., Arboleda-Velásquez, J.F., D’Amore, P.A. (2016). Coculture Assays for Endothelial Cells-Mural Cells Interactions. In: Ribatti, D. (eds) Tumor Angiogenesis Assays. Methods in Molecular Biology, vol 1464. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3999-2_4
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DOI: https://doi.org/10.1007/978-1-4939-3999-2_4
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