Abstract
Bioluminescence is a process that converts biochemical energy to visible light. Bioluminescence-based imaging technology has been widely applicable in the imaging of process envisioned in life sciences. As one of the most popular bioluminescence system, the firefly luciferin–luciferase system is exceptionally suitable for deep tissue imaging in living animals owing to its long wavelength emission light. Herein, we report the experimental detail of bioluminogenic imaging of aminopeptidase N activity both in vitro and in vivo.
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Acknowledgement
This work was supported by grants from the Program of New Century Excellent Talents in University (No. NCET-11-0306), the Shandong Natural Science Foundation (No. JQ201019), the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT13028), the Major Project of Science and Technology of Shandong Province (No. 2015ZDJS04001), the Shandong Key Research & Development Project (No. 2015GSF118166) and the Independent Innovation Foundation of Shandong University, IIFSDU (No. 2010JQ005 and 2014JC008). The first two authors contributed to this article equally.
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Wu, W., Chen, L., Li, J., Du, L., Li, M. (2016). Bioluminogenic Imaging of AminopeptidaseN In Vitro and In Vivo. In: Kim, S. (eds) Bioluminescence. Methods in Molecular Biology, vol 1461. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3813-1_7
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DOI: https://doi.org/10.1007/978-1-4939-3813-1_7
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