Skip to main content
SpringerLink
Log in

A Bioluminescence Assay System for Imaging Metal Cationic Activities in Urban Aerosols

  • Protocol
  • First Online:
Bioluminescence

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1461))

  • 1530 Accesses

Abstract

A bioluminescence-based assay system was fabricated for an efficient determination of the activities of air pollutants. The following four components were integrated into this assay system: (1) an 8-channel assay platform uniquely designed for simultaneously sensing multiple optical samples, (2) single-chain probes illuminating toxic chemicals or heavy metal cations from air pollutants, (3) a microfluidic system for circulating medium mimicking the human body, and (4) the software manimulating the above system. In the protocol, we briefly introduce how to integrate the components into the system and the application to the illumination of the metal cationic activities in air pollutants.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Fan F, Wood KV (2007) Bioluminescent assays for high-throughput screening. Assay Drug Dev Technol 5:127–136

    Article  CAS  PubMed  Google Scholar 

  2. Kim SB (2012) Labor-effective manipulation of marine and beetle luciferases for bioassays. Protein Eng Des Sel 25:261–269

    Article  CAS  PubMed  Google Scholar 

  3. Roda A, Guardigli M, Michelini E, Mirasoli M (2009) Bioluminescence in analytical chemistry and in vivo imaging. Trends Anal Chem 28:307–322

    Article  CAS  Google Scholar 

  4. Kim SB, Torimura M, Tao H (2013) Creation of artificial luciferases for bioassays. Bioconjug Chem 24:2067–2075

    Article  CAS  PubMed  Google Scholar 

  5. Kim SB, Izumi H (2014) Functional artificial luciferases as an optical readout for bioassays. Biochem Biophys Res Commun 448:418–423

    Article  CAS  PubMed  Google Scholar 

  6. Kim SB, Suzuki T, Kimura A (2013) A bioluminescent assay system for whole-cell determination of hormones. Chem Pharm Bull 61:706–713

    Article  CAS  PubMed  Google Scholar 

  7. Kim SB, Miller S, Suzuki N, Senda T, Nishihara R, Suzuki K (2015) Cation-driven optical properties of artificial luciferases. Anal Sci 31:955–960

    Article  CAS  PubMed  Google Scholar 

  8. Mitkus RJ, Powell JL, Zeisler R, Squibb KS (2013) Comparative physicochemical and biological characterization of NIST Interim Reference Material PM2.5 and SRM 1648 in human A549 and mouse RAW264.7 cells. Toxicol in Vitro 27:2289–2298

    Article  CAS  PubMed  Google Scholar 

  9. Kim SB, Sato M, Tao H (2008) Circularly permutated bioluminescent probes for illuminating ligand-activated protein dynamics. Bioconjugate Chem 19:2480–2486

    Article  CAS  Google Scholar 

  10. Yanagiya T, Imura N, Enomoto S, Kondo Y, Himeno S (2000) Suppression of a high-affinity transport system for manganese in cadmium-resistant metallothionein-null cells. J Pharmacol Exp Ther 292:1080–1086

    CAS  PubMed  Google Scholar 

Download references

Acknowledgement

This work was partly supported by JSPS KAKENHI Grant Numbers 26288088, 15KK0029, and 16K14051.

Author information

Authors and Affiliations

  1. Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST), Onogawa 16-1, Tsukuba, Ibaraki, 305-8569, Japan

    Sung-Bae Kim & Ryuichi Naganawa

  2. Nishihara Electronics co. ltd, 6-8-30, Nishihara, Kashiwa, Chiba, 277-0885, Japan

    Shingo Murata & Takayoshi Nakayama

  3. Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), 1-1 Oho, Tsukuba, Ibaraki, 305-0801, Japan

    Simon Miller & Toshiya Senda

Authors
  1. Sung-Bae Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ryuichi Naganawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shingo Murata
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Takayoshi Nakayama
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Simon Miller
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Toshiya Senda
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sung-Bae Kim .

Editor information

Editors and Affiliations

  1. Natl Inst of AIST Res Inst for Envt Mgt Tech, Tsukuba, Japan

    Sung Bae Kim

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer Science+Business Media New York

About this protocol

Cite this protocol

Kim, SB., Naganawa, R., Murata, S., Nakayama, T., Miller, S., Senda, T. (2016). A Bioluminescence Assay System for Imaging Metal Cationic Activities in Urban Aerosols. In: Kim, S. (eds) Bioluminescence. Methods in Molecular Biology, vol 1461. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3813-1_23

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-3813-1_23

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3811-7

  • Online ISBN: 978-1-4939-3813-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation