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Preparation and Assay of Simple Light off Biosensor Based on Immobilized Bioluminescent Bacteria for General Toxicity Assays

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Bioluminescence

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1461))

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Abstract

We describe a simple light off bioluminescent biosensor for general environmental toxicity assays based on immobilized bioluminescent bacteria engineered with beetle luciferases.

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Author information

Authors and Affiliations

  1. Laboratory of Biochemistry and Biotechnology of Bioluminescence, Department of Physics, Chemistry and Mathematics, Federal University of São Carlos (UFSCar), Sorocaba, SP, Brazil

    G. V. M. Gabriel & V. R. Viviani

  2. Graduate School of Biotechnology and Environmental Monitoring, Federal University of São Carlos (UFSCar), il. João Leme dos Santos Road, SP-264, Km 110, Sorocaba, SP, Brazil

    G. V. M. Gabriel & V. R. Viviani

  3. Graduate School of Evolutive Genetics and Molecular Biology, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil

    G. V. M. Gabriel & V. R. Viviani

Authors
  1. G. V. M. Gabriel
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  2. V. R. Viviani
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Corresponding author

Correspondence to V. R. Viviani .

Editor information

Editors and Affiliations

  1. Natl Inst of AIST Res Inst for Envt Mgt Tech, Tsukuba, Japan

    Sung Bae Kim

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Gabriel, G.V.M., Viviani, V.R. (2016). Preparation and Assay of Simple Light off Biosensor Based on Immobilized Bioluminescent Bacteria for General Toxicity Assays. In: Kim, S. (eds) Bioluminescence. Methods in Molecular Biology, vol 1461. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3813-1_18

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  • DOI: https://doi.org/10.1007/978-1-4939-3813-1_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3811-7

  • Online ISBN: 978-1-4939-3813-1

  • eBook Packages: Springer Protocols

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