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Skeletal Muscle Regeneration in the Mouse pp 321–336Cite as

Assessment of the Contractile Properties of Permeabilized Skeletal Muscle Fibers

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1460))

Abstract

Permeabilized individual skeletal muscle fibers offer the opportunity to evaluate contractile behavior in a system that is greatly simplified, yet physiologically relevant. Here we describe the steps required to prepare, permeabilize and preserve small samples of skeletal muscle. We then detail the procedures used to isolate individual fiber segments and attach them to an experimental apparatus for the purpose of controlling activation and measuring force generation. We also describe our technique for estimating the cross-sectional area of fiber segments. The area measurement is necessary for normalizing the absolute force to obtain specific force, a measure of the intrinsic force-generating capability of the contractile system.

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Acknowledgements

This work was supported by the following funding sources: R01-AG050676, R01-AR063649, F31-AR065931.

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Authors and Affiliations

  1. Department of Surgery, Section of Plastic Surgery, University of Michigan Medical School, Ann Arbor, MI, USA

    Dennis R. Claflin

  2. Department of Biomedical Engineering, University of Michigan College of Engineering, Ann Arbor, MI, USA

    Dennis R. Claflin & Susan V. Brooks

  3. Department of Orthopaedic Surgery, University of Michigan Medical School, Ann Arbor, MI, USA

    Stuart M. Roche, Jonathan P. Gumucio & Christopher L. Mendias

  4. Department of Molecular & Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI, USA

    Jonathan P. Gumucio, Christopher L. Mendias & Susan V. Brooks

Authors
  1. Dennis R. Claflin
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  2. Stuart M. Roche
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  3. Jonathan P. Gumucio
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  4. Christopher L. Mendias
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  5. Susan V. Brooks
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Corresponding author

Correspondence to Susan V. Brooks .

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Editors and Affiliations

  1. Lillehei Heart Institute, University of Minnesota, Minneapolis, Minnesota, USA

    Michael Kyba

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Claflin, D.R., Roche, S.M., Gumucio, J.P., Mendias, C.L., Brooks, S.V. (2016). Assessment of the Contractile Properties of Permeabilized Skeletal Muscle Fibers. In: Kyba, M. (eds) Skeletal Muscle Regeneration in the Mouse. Methods in Molecular Biology, vol 1460. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-3810-0_22

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  • DOI: https://doi.org/10.1007/978-1-4939-3810-0_22

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-3808-7

  • Online ISBN: 978-1-4939-3810-0

  • eBook Packages: Springer Protocols

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