Abstract
Volumetric muscle loss (VML) injury is prevalent in severe extremity trauma and is an emerging focus area among orthopedic and regenerative medicine fields. VML injuries are the result of an abrupt, frank loss of tissue and therefore of different etiology from other standard rodent injury models to include eccentric contraction, ischemia reperfusion, crush, and freeze injury. The current focus of many VML-related research efforts is to regenerate the lost muscle tissue and thereby improve muscle strength. Herein, we describe a VML model in the anterior compartment of the hindlimb that is permissible to repeated neuromuscular strength assessments and is validated in mouse, rat, and pig.
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Acknowledgements
All unpublished animal experiments were conducted in compliance with the Animal Welfare Act, the implementing Animal Welfare Regulations, and the principles of the Guide for the Care and Use of Laboratory Animals. This work was supported by Combat Casualty Care and Clinical and Rehabilitative Medicine Research Programs.
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The opinions or assertions contained herein are the private views of the author and are not be construed as official or as reflecting the views of the Department of the Army or the Department of Defense.
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Pollot, B.E., Corona, B.T. (2016). Volumetric Muscle Loss. In: Kyba, M. (eds) Skeletal Muscle Regeneration in the Mouse. Methods in Molecular Biology, vol 1460. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-3810-0_2
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DOI: https://doi.org/10.1007/978-1-4939-3810-0_2
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