Abstract
In vitro muscle contractile function assays are important to characterize the differences between different muscle types (e.g., slow vs. fast), between a diseased and non-diseased muscle, or importantly, to demonstrate the efficacy of a muscle treatment such as a drug, an overexpressed transgene, or knockout of a specific gene. Fundamental contractile properties can be assessed by twitch, tetanic, force–frequency, force–velocity, and fatigue assays. Many of these assays are conducted with the muscle at a constant length, e.g., an isometric contraction. However, to better represent the dynamic purpose of muscles in vivo (e.g., to move limbs), dynamic assays such as the force–velocity (concentric contractions) or stretch-injury (eccentric contractions) should also be obtained. Characterizing skeletal muscle function in vitro is a powerful approach to demonstrate efficacy of a treatment to rescue diseased muscle and to assess functional regeneration.
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References
Call JA, Lowe DA (2016) Eccentric contraction-induced muscle injury: reproducible, quantitative, physiological models to impair skeletal muscle’s capacity to generate force. In: Kyba M (ed) Skeletal muscle regeneration in the mouse. Springer, New York
Blaauw B, Schiaffino S, Reggiani C (2013) Mechanisms modulating skeletal muscle phenotype. Compr Physiol 3(4):1645–1687
Schiaffino S, Reggiani C (2011) Fiber types in mammalian skeletal muscles. Physiol Rev 91(4):1447–1531
Glaser BW et al (2010) Relative proportions of hybrid fibres are unaffected by 6 weeks of running exercise in mouse skeletal muscles. Exp Physiol 95(1):211–221
Luedeke JD et al (2004) Properties of slow- and fast-twitch skeletal muscle from mice with an inherited capacity for hypoxic exercise. Comp Biochem Physiol A Mol Integr Physiol 138(3):373–382
Quiat D et al (2011) Concerted regulation of myofiber-specific gene expression and muscle performance by the transcriptional repressor Sox6. Proc Natl Acad Sci U S A 108(25):10196–10201
Lynch GS et al (2001) Force and power output of fast and slow skeletal muscles from mdx mice 6–28 months old. J Physiol 535(Pt 2):591–600
Childers MK et al (2014) Gene therapy prolongs survival and restores function in murine and canine models of myotubular myopathy. Sci Transl Med 6(220):220ra10
Filareto A et al (2015) Pax3-induced expansion enables the genetic correction of dystrophic satellite cells. Skelet Muscle 5:36
McClung JM et al (2010) Overexpression of antioxidant enzymes in diaphragm muscle does not alter contraction-induced fatigue or recovery. Exp Physiol 95(1):222–231
Russell KA et al (2015) Mouse forepaw lumbrical muscles are resistant to age-related declines in force production. Exp Gerontol 65:42–45
Mendez J, Keys A (1960) Density and composition of mammalian muscle. Metabolism 9:184–188
Brooks SV, Faulkner JA (1988) Contractile properties of skeletal muscles from young, adult and aged mice. J Physiol 404:71–82
Head SI, Arber MB (2013) An active learning mammalian skeletal muscle lab demonstrating contractile and kinetic properties of fast- and slow-twitch muscle. Adv Physiol Educ 37(4):405–414
Asmussen G, Gaunitz U (1989) Temperature effects on isometric contractions of slow and fast twitch muscles of various rodents—dependence on fibre type composition: a comparative study. Biomed Biochim Acta 48(5–6):S536–S541
Graber TG et al (2015) C57BL/6 life span study: age-related declines in muscle power production and contractile velocity. Age (Dordr) 37(3):9773
Allen DG, Lamb GD, Westerblad H (2008) Skeletal muscle fatigue: cellular mechanisms. Physiol Rev 88(1):287–332
Call JA et al (2008) Endurance capacity in maturing mdx mice is markedly enhanced by combined voluntary wheel running and green tea extract. J Appl Physiol (1985) 105(3):923–932
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Sperringer, J.E., Grange, R.W. (2016). In Vitro Assays to Determine Skeletal Muscle Physiologic Function. In: Kyba, M. (eds) Skeletal Muscle Regeneration in the Mouse. Methods in Molecular Biology, vol 1460. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-3810-0_19
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DOI: https://doi.org/10.1007/978-1-4939-3810-0_19
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