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Unconventional Protein Secretion pp 249–257Cite as

Vacuum Infiltration-Centrifugation Method for Apoplastic Protein Extraction in Grapevine

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1459))

Abstract

The apoplastic fluid moving in the extracellular space external to the plasma membrane provides a means of delivering molecules and facilitates intercellular communications. However, the apoplastic fluid extraction from in planta systems remains challenging and this is particularly true for grapevine (Vitis vinifera L.), a worldwide-cultivated fruit plant. Here, we describe an optimized vacuum-infiltration-centrifugation method to extract soluble proteins from apoplastic fluid of grapevine leaves. This optimized method allows recovering of the grapevine apoplastic soluble proteins suitable for mono- and bi-dimensional gel electrophoresis for further proteomic analysis in order to elucidate their physiological functions.

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Acknowledgment

The authors are grateful to Vranken Pommery (Reims, France) for access to their vineyard. This study was supported by the “Region Champagne Ardennes” and by “Comité Champagne” (Epernay, France) through the project VINEAL 2 for the Ph.D. grant of B.D. and by AROCU (Association Recherche Oenologique Champagne et Université).

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Authors and Affiliations

  1. Laboratoire Stress, Défenses et Reproduction des Plantes, Unité de Recherche Vignes et Vins de Champagne-EA 4707, SFR Condorcet – FR CNRS 3417, Université de Reims Champagne-Ardenne, Moulin de la Housse, BP 1039, 51687, Reims Cedex 2, France

    Bertrand Delaunois, Fabienne Baillieul, Christophe Clément, Philippe Jeandet & Sylvain Cordelier

Authors
  1. Bertrand Delaunois
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  2. Fabienne Baillieul
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  3. Christophe Clément
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  4. Philippe Jeandet
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  5. Sylvain Cordelier
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Corresponding author

Correspondence to Sylvain Cordelier .

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Editors and Affiliations

  1. Instituto di Bioscienze e Biorisorse, Perugia, Italy

    Andrea Pompa

  2. Instituto di Bioscienze e Biorisorse, Perugia, Italy

    Francesca De Marchis

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Delaunois, B., Baillieul, F., Clément, C., Jeandet, P., Cordelier, S. (2016). Vacuum Infiltration-Centrifugation Method for Apoplastic Protein Extraction in Grapevine. In: Pompa, A., De Marchis, F. (eds) Unconventional Protein Secretion. Methods in Molecular Biology, vol 1459. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3804-9_17

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  • DOI: https://doi.org/10.1007/978-1-4939-3804-9_17

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3802-5

  • Online ISBN: 978-1-4939-3804-9

  • eBook Packages: Springer Protocols

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