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Laser Capture Microdissection as a Tool to Study Tumor Stroma

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The Tumor Microenvironment

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1458))

Abstract

Laser capture microdissection (or LCM) allows for isolation of cells from specific tissue compartments, which can then be followed by DNA, RNA, and/or protein isolation and downstream characterization. Unlike other methods for cell isolation, LCM can be directed towards cells situated in specific anatomical contexts, and is therefore of significant value when investigating the tumor microenvironment, where localization is often key to function. Here, we present a summary of ways in which LCM can be utilized, as well as protocols for the isolation of tumor and tumor-associated stromal elements from frozen breast cancer samples, with a focus on preparation of samples for RNA characterization.

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Acknowledgements

This work was supported by funding from the Database and Tissue Bank Axis of the “Réseau de Recherche sur le Cancer” of the “Fonds de Recherche du Québec-Santé” and the Québec Breast Cancer Foundation to M.P.

Author information

Authors and Affiliations

  1. Rosalind and Morris Goodman Cancer Research Centre, McGill University, Montréal, QC, Canada

    Nicholas R. Bertos & Morag Park

  2. Department of Experimental Medicine, McGill University, Montréal, QC, Canada

    Morag Park

  3. Department of Biochemistry, McGill University, Montréal, QC, Canada

    Morag Park

  4. Department of Oncology, McGill University, Montréal, QC, Canada

    Morag Park

  5. Department of Pathology, McGill University, Montréal, QC, Canada

    Morag Park

Authors
  1. Nicholas R. Bertos
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  2. Morag Park
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Correspondence to Morag Park .

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Editors and Affiliations

  1. Lady Davis Institute for Medical Research, Montreal, Québec, Canada

    Josie Ursini-Siegel

  2. Goodman Cancer Research Centre, McGill University, Montreal, Québec, Canada

    Nicole Beauchemin

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Bertos, N.R., Park, M. (2016). Laser Capture Microdissection as a Tool to Study Tumor Stroma. In: Ursini-Siegel, J., Beauchemin, N. (eds) The Tumor Microenvironment. Methods in Molecular Biology, vol 1458. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3801-8_2

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  • DOI: https://doi.org/10.1007/978-1-4939-3801-8_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3799-8

  • Online ISBN: 978-1-4939-3801-8

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