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The Tumor Microenvironment pp 261–271Cite as

CRISPR/Cas9 Genome Editing as a Strategy to Study the Tumor Microenvironment in Transgenic Mice

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1458))

Abstract

Development of engineered site-specific endonucleases like zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9 has been revolutionizing genetic approaches in biomedical research fields. These new tools have opened opportunities to carry out targeted genome editing in mouse zygotes without the need for manipulating embryonic stem cells, which have a higher technical burden and many constraints in strain availability. Specific genetic modifications can be directly generated in working genetic backgrounds. This new approach saves time and costs associated with generation and backcrossing of genetically modified animals and will facilitate their use in various cancer research fields.

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Acknowledgements

I thank Aaron Kwong and Nobuko Yamanaka for valuable comments. I also thank the past and present members of Yamanaka lab and the Goodman Cancer Research Centre Transgenic facility for optimizing our protocols. Y.Y. is supported by CIHR (MOP111197) and NSERC (RGPIN418720).

Author information

Authors and Affiliations

  1. GCRC Transgenic Facility, Department of Human Genetics, Goodman Cancer Research Centre, McGill University, 1160 Pine Avenue West, rm419, Montreal, QC, Canada, H3A1A3

    Yojiro Yamanaka

Authors
  1. Yojiro Yamanaka
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Corresponding author

Correspondence to Yojiro Yamanaka .

Editor information

Editors and Affiliations

  1. Lady Davis Institute for Medical Research, Montreal, Québec, Canada

    Josie Ursini-Siegel

  2. Goodman Cancer Research Centre, McGill University, Montreal, Québec, Canada

    Nicole Beauchemin

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© 2016 Springer Science+Business Media New York

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Yamanaka, Y. (2016). CRISPR/Cas9 Genome Editing as a Strategy to Study the Tumor Microenvironment in Transgenic Mice. In: Ursini-Siegel, J., Beauchemin, N. (eds) The Tumor Microenvironment. Methods in Molecular Biology, vol 1458. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3801-8_19

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  • DOI: https://doi.org/10.1007/978-1-4939-3801-8_19

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3799-8

  • Online ISBN: 978-1-4939-3801-8

  • eBook Packages: Springer Protocols

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