Abstract
Oogenesis is a fundamental biological process for the transmission of genetic information to the next generations. Drosophila has proven to be a valuable model for elucidating the molecular and cellular mechanisms involved in this developmental process. It has been shown that autophagy participates in the maturation of the egg chamber. Here we provide a protocol for monitoring and quantification of the autophagic process in the Drosophila germline cells using the fluorescent reporters mCherry-DmAtg8a and GFP-mCherry-DmAtg8a.
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Acknowledgments
This work is supported by a BBSRC grant (BB/L006324/1) awarded to Dr. Ioannis P. Nezis.
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Jacomin, AC., Nezis, I.P. (2016). Using Fluorescent Reporters to Monitor Autophagy in the Female Germline Cells in Drosophila melanogaster . In: Nezis, I. (eds) Oogenesis. Methods in Molecular Biology, vol 1457. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3795-0_5
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DOI: https://doi.org/10.1007/978-1-4939-3795-0_5
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