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Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles

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Multipotent Stem Cells of the Hair Follicle

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1453))

Abstract

The hair follicle undergoes lifelong cycling and growth. Previous studies have been focused on epithelial stem cells in the hair follicles. Neural crest stem cells (NCSCs) are pluripotent cells that can persist in adult tissues. We have previously demonstrated that human NCSCs can be isolated from hair follicles. Here, we present a protocol to isolate NCSCs from human hair follicles based on their specific surface-marker expression of CD271/HNK1 or CD271/CD49D (alpha4 integrin). NCSCs can be expanded in the culture as neural spheres or attached cells.

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Acknowledgments

This work was supported by NIH grants R01AR054593 and R01AR054593-S1 to X. Xu.

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Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, 19104, USA

    Ruifeng Yang & Xiaowei Xu M.D., Ph.D.

Authors
  1. Ruifeng Yang
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  2. Xiaowei Xu M.D., Ph.D.
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Corresponding author

Correspondence to Xiaowei Xu M.D., Ph.D. .

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Editors and Affiliations

  1. University of California, AntiCancer, Inc., San Diego, California, USA

    Robert M Hoffman

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© 2016 Springer Science+Business Media New York

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Yang, R., Xu, X. (2016). Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles. In: Hoffman, R. (eds) Multipotent Stem Cells of the Hair Follicle. Methods in Molecular Biology, vol 1453. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3786-8_7

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  • DOI: https://doi.org/10.1007/978-1-4939-3786-8_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3784-4

  • Online ISBN: 978-1-4939-3786-8

  • eBook Packages: Springer Protocols

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