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Nestin-Based Reporter Transgenic Mouse Lines

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Multipotent Stem Cells of the Hair Follicle

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1453))

Abstract

Nestin expression marks stem and progenitor cells of the neural lineage. Transgenic mouse lines, originally generated to identify neural stem cells, can also help to identify, track, and isolate stem and progenitor cells in a range of tissues of the ectodermal, endodermal, and mesodermal origin. Here, we describe the generation of transgenic mouse lines expressing fluorescent proteins (FP) under the control of critical regulatory elements of the nestin gene and their use for identifying and analyzing adult stem and progenitor cells in various tissues.

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Authors and Affiliations

  1. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724, USA

    John Mignone, Natalia Peunova & Grigori Enikolopov Ph.D.

Authors
  1. John Mignone
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  2. Natalia Peunova
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  3. Grigori Enikolopov Ph.D.
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Corresponding author

Correspondence to Grigori Enikolopov Ph.D. .

Editor information

Editors and Affiliations

  1. University of California, AntiCancer, Inc., San Diego, California, USA

    Robert M Hoffman

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Mignone, J., Peunova, N., Enikolopov, G. (2016). Nestin-Based Reporter Transgenic Mouse Lines. In: Hoffman, R. (eds) Multipotent Stem Cells of the Hair Follicle. Methods in Molecular Biology, vol 1453. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3786-8_2

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  • DOI: https://doi.org/10.1007/978-1-4939-3786-8_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3784-4

  • Online ISBN: 978-1-4939-3786-8

  • eBook Packages: Springer Protocols

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