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Using High-Speed Chronoamperometry to Measure Biogenic Amine Release and Uptake In Vivo

  • Lynette C. DawsEmail author
  • W. Anthony Owens
  • Glenn M. Toney
Protocol
Part of the Neuromethods book series (NM, volume 118)

Abstract

Here, we describe the method of high-speed chronoamperometry and its application for measuring release and clearance of biogenic amine neurotransmitters (serotonin, dopamine, and norepinephrine) in the intact and living mammalian brain. Chronoamperometry belongs to a family of electrochemical techniques collectively known as voltammetry, the only techniques currently available for “real-time” measurement of neurotransmitter transporter activity in vivo. Because of the small size of recording electrodes (<30 μm) and the relatively rapid sampling rate (sub-second), these techniques can be used to quantify release and clearance kinetics for biogenic amines in discrete brain regions. Chronoamperometry has been effectively used to study the impact of drugs, various environmental influences (e.g. stress), the estrous cycle, and age, among other stimuli, on the function of biogenic amine transporters in vivo.

A major part of performing high-speed chronoamperometry is the preparatory work, including fabricating and calibrating carbon fiber electrodes, creating electrode-micropipette assemblies and stereotaxically implanting them in brain. Details for all steps are provided here, including how to histologically verify electrode placement at the conclusion of recordings. Chronoamperometry provides a unique window to “view” biogenic amine transporter function in the living animal.

Key words

Chronoamperometry In vivo electrochemistry Carbon fiber electrode Serotonin Dopamine Norepinephrine Biogenic amine transporters 

Notes

Acknowledgments

Studies described herein were funded in part by NIH grants MH64489, MH093320, MH106978, DA18992, and DA014684 to L.C.D. and NIH grants HL102310 and HL088052 to G.M.T. The authors gratefully acknowledge Lester Rosebrock for photography.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Lynette C. Daws
    • 1
    Email author
  • W. Anthony Owens
    • 1
  • Glenn M. Toney
    • 1
  1. 1.Department of PhysiologyUniversity of Texas Health Science Center at San AntonioSan AntonioUSA

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