Organic Cation Transport Measurements Using Fluorescence Techniques
Analysis of transport processes using fluorescent substrates is a powerful tool to dynamically study various aspects of several transporters. The fluorescent organic cation 4(4-dimethylaminostyryl)-N-methylpyridinium (ASP+) due to its specific fluorescence properties is a valuable probe for studying transport mediated by transporters of organic cations. ASP+ is accepted by many members of the family of organic cation transporters and can be utilized in a wide spectrum of experimental settings analyzing such transport dynamics from the in vitro to the in vivo situation. Since its first introduction in 1994, ASP+ has been widely used by several laboratories worldwide for all kinds of studies analyzing organic cation transport.
Key wordsASP+ Organic cation transport OCT Proximal tubule Microtiter plate reader Epifluorescence Fluorescence imaging Two-photon microscopy Expression systems
The work from the authors’ laboratory described here was supported by the German Research Council (Schl 277/8-1 to 8-4 and 12-3 and CI 107/4-1 to 4-3), the German Krebshilfe Foundation (#108539), the Interdisciplinary Center of Clinical Research (IZKF, Cia2/013/13), and the Innovative Medical Research (IMF) of the Medical Faculty of the University Münster, Germany.
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