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Classical Radioligand Uptake and Binding Methods in Transporter Research: An Emphasis on the Monoamine Neurotransmitter Transporters

  • Sonja SucicEmail author
  • Heinz Bönisch
Protocol
Part of the Neuromethods book series (NM, volume 118)

Abstract

Radioligand uptake and binding assays denote an invaluable tool in the field of neurotransmitter transporter research. Their benefits have been evident since the late 1950s, and they continue to contribute major insights into transporter function and structure to date. In the current chapter, we focus primarily on the family of monoamine (MA) neurotransmitter transporters (MATs), i.e., transporters (T) for norepinephrine [noradrenaline] (NET), dopamine (DAT), and serotonin [5-hydroxy tryptamine, 5-HT] (SERT), dysfunction of which has been linked to numerous neuropsychiatric disorders and substance abuse. Radiotracer assays have provided a major way of elucidating the mechanisms of action of not only endogenous substrates (e.g., NE, SER, and DA), but also many diverse substances such as antidepressants (e.g., imipramine and citalopram), psychostimulants (e.g., amphetamine and cocaine), toxins (e.g., conotoxins), or neurotoxins (e.g., 1-methyl-4-phenylpyridinium, MPP+) that exert their action on MATs. In this chapter we describe the basic principles and experimental procedures of radiotracer assays commonly used in the studies of MATs.

Key words

Radioligands Uptake Binding Monoamine neurotransmitter transporters 

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© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Institute of Pharmacology, Center of Physiology and PharmacologyMedical University of ViennaViennaAustria
  2. 2.Biomedical Center, Institute of Pharmacology and ToxicologyUniversity of BonnBonnGermany

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