Abstract
Isobaric tags for relative and absolute quantitation (iTRAQ) is a technology that utilizes isobaric reagents to label the primary amines of peptides and proteins and is used in proteomics to study quantitative changes in the proteome by tandem mass spectrometry. Here, we present an adaptation of the iTRAQ experimental protocol for plants that allows the identification and quantitation of more than 12,000 plant proteins in Arabidopsis with a false discovery rate of less than 5 %.
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Acknowledgement
Work in the Schmidt lab is supported by MoST and Academia Sinica.
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Vélez-Bermúdez, I.C., Wen, TN., Lan, P., Schmidt, W. (2016). Isobaric Tag for Relative and Absolute Quantitation (iTRAQ)-Based Protein Profiling in Plants. In: Lois, L., Matthiesen, R. (eds) Plant Proteostasis. Methods in Molecular Biology, vol 1450. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3759-2_17
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DOI: https://doi.org/10.1007/978-1-4939-3759-2_17
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-3759-2
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