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Expression, Purification, and Enzymatic Analysis of Plant SUMO Proteases

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Plant Proteostasis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1450))

Abstract

The conjugation of SUMO can profoundly change the behavior of substrate proteins, impacting a wide variety of cellular responses. SUMO proteases are emerging as key regulators of plant adaptation to its environment because of their instrumental role in the SUMO deconjugation process. Here, we describe how to express, purify, and determine SUMO deconjugation activity of a plant SUMO protease.

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References

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Acknowledgment

European Research council provided grant-aided support to AS in the form of an ERC consolidator award.

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Authors and Affiliations

  1. School of Biological and Biomedical Sciences, University of Durham, Durham, DH1 3LE, UK

    Gary Yates, Anjil Srivastava, Beatriz Orosa & Ari Sadanandom

Authors
  1. Gary Yates
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  2. Anjil Srivastava
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  3. Beatriz Orosa
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  4. Ari Sadanandom
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Corresponding author

Correspondence to Ari Sadanandom .

Editor information

Editors and Affiliations

  1. Development Program, CRAG (CSIC-IRTA-UAB-UB) Edifici CRAGCampus, UAB Bellaterra (Cerdanyola del Vallés), Barcelona, Spain

    L. Maria Lois

  2. Computational and Experimental Biology Group, National Health Institute Doctor Ricardo Jorge, IP, Lisbon, Portugal

    Rune Matthiesen

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© 2016 Springer Science+Business Media New York

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Yates, G., Srivastava, A., Orosa, B., Sadanandom, A. (2016). Expression, Purification, and Enzymatic Analysis of Plant SUMO Proteases. In: Lois, L., Matthiesen, R. (eds) Plant Proteostasis. Methods in Molecular Biology, vol 1450. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3759-2_10

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  • DOI: https://doi.org/10.1007/978-1-4939-3759-2_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3757-8

  • Online ISBN: 978-1-4939-3759-2

  • eBook Packages: Springer Protocols

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