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The Nuclear Receptor Superfamily pp 105–117Cite as

Posttranslational Modifications of Steroid Receptors: Phosphorylation

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1443))

Abstract

The detection of phosphorylation status of proteins has become a critical component of the analysis of activity, localization, and turnover studies of most proteins, particularly for those involved in signaling. The androgen receptor is no exception to this rule with its localization, transcriptional activity, and interactions determined by a series of key phosphorylations on serine residues. Here we have presented a series of techniques for the investigation of the phosphorylation status and intracellular localization of the androgen receptor after hormone and growth factor stimulation of cells in culture (in vitro) and in prostate cancer tissue (in vivo). Modified methods for immunohistochemistry, immunoblotting and immunofluorescence detection with high efficacy for the measurement and monitoring of androgen receptor are presented here alongside examples of their use.

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Acknowledgements

This work was carried out at the former MRC Human Reproductive Sciences Unit, Edinburgh. The work was supported by a visiting scientist grant (I.J.M.) and postdoctoral fellowship (D.McG.) from the Medical Research Council, UK. We are grateful to Dr. Axel Thomson for providing tissue samples.

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Authors and Affiliations

  1. Wolfson Wohl Translational Research Centre, Institute of Cancer Sciences, University of Glasgow, Garscube Estate, Swithback Road, Glasgow, G61 1QH, UK

    Dagmara McGuinness

  2. School of Medicine, Medical Sciences and Nutrition, Institute of Medical Sciences, University of Aberdeen, Foresthill, Aberdeen, AB25 2ZD, UK

    Iain J. McEwan

Authors
  1. Dagmara McGuinness
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  2. Iain J. McEwan
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Correspondence to Iain J. McEwan .

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Editors and Affiliations

  1. Ins. of Med. Sci. Uni. of Aberdeen, Sch. of Medicine Med. Sci. and Nutrition, Aberdeen, United Kingdom

    Iain J. McEwan, PhD

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McGuinness, D., McEwan, I.J. (2016). Posttranslational Modifications of Steroid Receptors: Phosphorylation. In: McEwan, PhD, I. (eds) The Nuclear Receptor Superfamily. Methods in Molecular Biology, vol 1443. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3724-0_7

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  • DOI: https://doi.org/10.1007/978-1-4939-3724-0_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3722-6

  • Online ISBN: 978-1-4939-3724-0

  • eBook Packages: Springer Protocols

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