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Measuring the Expression of microRNAs Regulated by Androgens

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The Nuclear Receptor Superfamily

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1443))

Abstract

The discovery of microRNAs (miRNAs) provided yet another mechanism of gene expression regulation. miRNAs have recently been also implicated in many diseases, including prostate cancer (PC). As PC is a highly androgen-dependent disease, extensive effort has been invested to identify the miRNAs that are androgen regulated. However, relatively few of them have been shown to be directly androgen regulated in PC. In this chapter we introduce the commonly used techniques to study the androgen regulation of miRNAs. The most cost-effective tool to profile global miRNA expression is microarray-based hybridization, whereas real-time quantitative reverse transcription PCR (qRT-PCR) is commonly used for the study of individual miRNAs.

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Authors and Affiliations

  1. Prostate Cancer Research Center, Institute of Biosciences and Medical Technology—BioMediTech and Fimlab Laboratories, University of Tampere and Tampere University Hospital, Tampere, Finland

    Mauro Scaravilli, Kati Kivinummi, Tapio Visakorpi & Leena Latonen

  2. Institute of Biosciences and Medical Technology, BioMediTech University of Tampere, Biokatu 6, 33520, Tampere, Finland

    Leena Latonen

Authors
  1. Mauro Scaravilli
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  2. Kati Kivinummi
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  3. Tapio Visakorpi
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  4. Leena Latonen
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Corresponding author

Correspondence to Leena Latonen .

Editor information

Editors and Affiliations

  1. Ins. of Med. Sci. Uni. of Aberdeen, Sch. of Medicine Med. Sci. and Nutrition, Aberdeen, United Kingdom

    Iain J. McEwan, PhD

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Scaravilli, M., Kivinummi, K., Visakorpi, T., Latonen, L. (2016). Measuring the Expression of microRNAs Regulated by Androgens. In: McEwan, PhD, I. (eds) The Nuclear Receptor Superfamily. Methods in Molecular Biology, vol 1443. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3724-0_10

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  • DOI: https://doi.org/10.1007/978-1-4939-3724-0_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3722-6

  • Online ISBN: 978-1-4939-3724-0

  • eBook Packages: Springer Protocols

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