Abstract
RNA interference is an invaluable tool in biology to specifically silence a given gene. Synthetic duplexes of RNA oligonucleotides are widely used to induce mRNA degradation in cultured cells or in whole organisms. They have to be vectorized to reach their target site. Here, we describe the preparation of highly efficient siRNA vectors based on cationic liposomes and polyanionic polymers and their application in cultured cells to silence reporter and/or endogenous genes.
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Acknowledgement
This work was supported by ANRT, Oséo Innovation and ANR.
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Arruda, D.C., Schlegel, A., Bigey, P., Escriou, V. (2016). Lipoplexes Strengthened by Anionic Polymers: Easy Preparation of Highly Effective siRNA Vectors Based on Cationic Lipids and Anionic Polymers. In: Candiani, G. (eds) Non-Viral Gene Delivery Vectors. Methods in Molecular Biology, vol 1445. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3718-9_8
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DOI: https://doi.org/10.1007/978-1-4939-3718-9_8
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3716-5
Online ISBN: 978-1-4939-3718-9
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