Abstract
The delivery of nucleic acids into cells remains an important laboratory cell culture technique and potential clinical therapy, based upon the initial cellular uptake, then translation into protein (in the case of DNA), or gene deletion by RNA interference (RNAi). Although viral delivery vectors are more efficient, the high production costs, limited cargo capacity, and the potential for clinical adverse events make nonviral strategies attractive. Cationic lipids are the most widely applied and studied nonviral vectors; however, much remains to be solved to overcome limitations of these systems. Advances in the field of cationic lipid-based nucleic acid (lipoplex) delivery rely upon the development of robust and reproducible lipoplex formulations, together with the use of cell culture assays. This chapter provides detailed protocols towards the formulation, delivery, and assessment of in vitro cationic lipid-based delivery of DNA.
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Acknowledgement
This work was supported in part by the British Council (PMI2 Gulf States Cooperation Grant No. RCGS206), the Biomedical Research Program intramural funding at Weill Cornell Medical College in Qatar, and the Qatar National Research Fund under the National Priorities Research Program, award NPRP08-705-3-144. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the Qatar National Research Fund.
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Jubeli, E., Goldring, W.P.D., Pungente, M.D. (2016). Cationic Lipid-Based Nucleic Acid Vectors. In: Candiani, G. (eds) Non-Viral Gene Delivery Vectors. Methods in Molecular Biology, vol 1445. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3718-9_2
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DOI: https://doi.org/10.1007/978-1-4939-3718-9_2
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