Abstract
Polyplexes are one of the most important and promising approaches to deliver exogenous DNA into cells. However, it is severely restricted by the aggregation of polyplexes. Surface-tethered polyplexes can inhibit the aggregation effect and increase the local concentrations of DNA, exhibiting an excellent potential in gene transfection. Since silicon nanowires have the ability to penetrate the cell membrane, branched polyethylenimine (bPEI)-grafted silicon nanowire arrays (SiNWAs) can stimulate gene transfection to a great extent. Herein, the method for the preparation of bPEI-grafted SiNWAs, as an example of surface-tethered polyplexes, is introduced in detail.
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Acknowledgement
This work was supported by the National Natural Science Foundation of China (21334004, 21374070 and 21474072), and the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (13KJA430006).
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Wang, H., Pan, J., Chen, H., Yuan, L. (2016). Application of Polyethylenimine-Grafted Silicon Nanowire Arrays for Gene Transfection. In: Candiani, G. (eds) Non-Viral Gene Delivery Vectors. Methods in Molecular Biology, vol 1445. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3718-9_18
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DOI: https://doi.org/10.1007/978-1-4939-3718-9_18
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