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From Artificial Amino Acids to Sequence-Defined Targeted Oligoaminoamides

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Non-Viral Gene Delivery Vectors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1445))

Abstract

Artificial oligoamino acids with appropriate protecting groups can be used for the sequential assembly of oligoaminoamides on solid-phase. With the help of these oligoamino acids multifunctional nucleic acid (NA) carriers can be designed and produced in highly defined topologies. Here we describe the synthesis of the artificial oligoamino acid Fmoc-Stp(Boc3)-OH, the subsequent assembly into sequence-defined oligomers and the formulation of tumor-targeted plasmid DNA (pDNA) polyplexes.

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Acknowledgements

This work was supported by the German Research Foundation (DFG) Excellence Cluster ″Nanosystems Initiative Munich″ and DFG Collaborative Research Center SFB824. We thank Wolfgang Rödl and Miriam Höhn for technical support, and Olga Brück for skillful assistance.

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Authors and Affiliations

  1. Pharmaceutical Biotechnology, Center for System-based Drug Research, Ludwig-Maximilians-University Munich, Butenandtstrasse 5-13, Munich, Germany

    Stephan Morys, Ernst Wagner & Ulrich Lächelt

  2. Nanosystems Initiative, Munich, Germany

    Ernst Wagner & Ulrich Lächelt

Authors
  1. Stephan Morys
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  2. Ernst Wagner
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  3. Ulrich Lächelt
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Corresponding author

Correspondence to Ulrich Lächelt .

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Editors and Affiliations

  1. Polytechnic University of Milan, Milan, Italy

    Gabriele Candiani

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Morys, S., Wagner, E., Lächelt, U. (2016). From Artificial Amino Acids to Sequence-Defined Targeted Oligoaminoamides. In: Candiani, G. (eds) Non-Viral Gene Delivery Vectors. Methods in Molecular Biology, vol 1445. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3718-9_15

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  • DOI: https://doi.org/10.1007/978-1-4939-3718-9_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3716-5

  • Online ISBN: 978-1-4939-3718-9

  • eBook Packages: Springer Protocols

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