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MicroRNA Profiling from RSV-Infected Biofluids, Whole Blood, and Tissue Samples

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Human Respiratory Syncytial Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1442))

Abstract

Several studies have shown that respiratory syncytial virus (RSV) can modulate the host innate immune response by dysregulation of host microRNAs (miRNAs) related to the antiviral response, a feature that also affects the memory immune response to RSV (Thornburg et al. MBio 3(6), 2012). miRNAs are small, endogenous, noncoding RNAs that function in posttranscriptional gene regulation. Here, we explain a compilation of methods for the purification, quantification, and characterization of miRNA expression profiles in biofluids, whole blood samples, and tissue samples obtained from in vivo studies. In addition, this chapter describes methods for the isolation of exosomal miRNA populations. Understanding alterations in miRNA expression profiles and identifying miRNA targets genes, and their contribution to the pathogenesis of RSV, may help elucidate novel mechanism of host-virus interaction (Rossi et al., Pediatr Pulmonol, 2015).

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Authors and Affiliations

  1. Animal Health Research Center, Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, 111 Carlton Street, Athens, GA, 30602, USA

    Lydia Anderson, Patricia A. Jorquera Ph.D. & Ralph A. Tripp Ph.D.

Authors
  1. Lydia Anderson
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  2. Patricia A. Jorquera Ph.D.
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  3. Ralph A. Tripp Ph.D.
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Corresponding author

Correspondence to Ralph A. Tripp Ph.D. .

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Editors and Affiliations

  1. College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA

    Ralph A. Tripp

  2. Department of Infectious Diseases, University of Georgia College of Veterinary Medicine, Athens, Georgia, USA

    Patricia A. Jorquera

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Anderson, L., Jorquera, P.A., Tripp, R.A. (2016). MicroRNA Profiling from RSV-Infected Biofluids, Whole Blood, and Tissue Samples. In: Tripp, R., Jorquera, P. (eds) Human Respiratory Syncytial Virus. Methods in Molecular Biology, vol 1442. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3687-8_14

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  • DOI: https://doi.org/10.1007/978-1-4939-3687-8_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3685-4

  • Online ISBN: 978-1-4939-3687-8

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