Abstract
Gene silencing through siRNA is an effective experimental tool to unravel molecular mechanisms involved in cellular processes. Here we describe a method to silence gene expression in primary human natural killer (NK) cells by transfecting ON-TARGETplus SMART pool siRNA using an electroporation-based method called Nucleofection®. The technique yields effective silencing of the target gene without any off-target effects.
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© 2016 Springer Science+Business Media New York
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Phatarpekar, P.V., Lee, D.A., Somanchi, S.S. (2016). Electroporation of siRNA to Silence Gene Expression in Primary NK Cells. In: Somanchi, S. (eds) Natural Killer Cells. Methods in Molecular Biology, vol 1441. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3684-7_22
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DOI: https://doi.org/10.1007/978-1-4939-3684-7_22
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3682-3
Online ISBN: 978-1-4939-3684-7
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