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Application of Fluorescence Polarization in HTS Assays

  • Xinyi HuangEmail author
  • Ann Aulabaugh
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1439)

Abstract

Steady-state measurements of fluorescence polarization have been widely adopted in the field of high-throughput screening for the study of biomolecular interactions. This chapter reviews the basic theory of fluorescence polarization, the underlying principle for using fluorescence polarization to study interactions between small-molecule fluorophores and macromolecular targets, and representative applications of fluorescence polarization in high-throughput screening.

Key words

FP Polarization Anisotropy Competition binding High-throughput screening 

Notes

Acknowledgments

The authors would like to thank Ray Unwalla of Wyeth Research for molecular modeling of E-76 and the E-76 based probes, and would like to thank Rebecca Shirk and Belew Mekonnen of Wyeth Research for collaboration on the TF/FVIIa, and would like to thank Shannon Stahler, Nina Kadakia, Gary Kalgaonkar, William Martin, Mariya Gazumyan, Pedro Sobers, and Jim LaRocque of Wyeth Research for contribution to the NR project, and would like to thank Richard Harrison of Wyeth Research for critical review of the chapter.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Immunology, Inflammation and Infectious Diseases Discovery and Translational Area Roche Pharma Research & Early DevelopmentRoche Innovation Center Shanghai, Roche R&D Center (China) LtdPudongChina

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