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Design and Implementation of High-Throughput Screening Assays

  • David J. PowellEmail author
  • Robert P. Hertzberg
  • Ricardo Macarrόn
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1439)

Abstract

HTS remains at the core of the drug discovery process, and so it is critical to design and implement HTS assays in a comprehensive fashion involving scientists from the disciplines of biology, chemistry, engineering, and informatics. This requires careful consideration of many options and variables, starting with the choice of screening strategy and ending with the discovery of lead compounds. At every step in this process, there are decisions to be made that can greatly impact the outcome of the HTS effort, to the point of making it a success or a failure. Although specific guidelines should be established to ensure that the screening assay reaches an acceptable level of quality, many choices require pragmatism and the ability to compromise opposing forces.

Key words

Bioassay Phenotypic Drug screening Human Methodology 

Notes

Acknowledgements

The authors are grateful to the many colleagues at GlaxoSmithKline past and present who helped over the years to shape the screening process and to build the collective knowledge succinctly described in this introduction.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • David J. Powell
    • 1
    Email author
  • Robert P. Hertzberg
    • 2
  • Ricardo Macarrόn
    • 3
  1. 1.Alternative Drug DiscoveryGSK PharmaceuticalsHertsUK
  2. 2.GSK Pharmaceuticals R&DPhiladelphiaUSA
  3. 3.Alternative Drug DiscoveryGSK PharmaceuticalsUpper ProvidenceUSA

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