Abstract
The propagation and titration of viruses are key virological techniques. Unlike other flaviviruses, such as the dengue viruses, West Nile virus (WNV) grows and plaques very efficiently on Vero cells, usually inducing strong cytopathic effect (CPE) and forming clear plaques. Here, we outline the steps for propagating WNV from culture supernatant stocks and homogenized organ/mosquito samples, as well as for determining virus titers in samples by serial-dilution plaque assay using neutral red or crystal violet stains.
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Acknowledgements
A.J.M. has been supported by a James W. McLaughlin Predoctoral Fellowship and a Jeane B. Kempner Predoctoral Scholarship.
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McAuley, A.J., Beasley, D.W.C. (2016). Propagation and Titration of West Nile Virus on Vero Cells. In: Colpitts, T. (eds) West Nile Virus. Methods in Molecular Biology, vol 1435. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3670-0_3
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DOI: https://doi.org/10.1007/978-1-4939-3670-0_3
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-3670-0
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