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West Nile Virus pp 175–206Cite as

Detection Protocols for West Nile Virus in Mosquitoes, Birds, and Nonhuman Mammals

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1435))

Abstract

West Nile virus is the most widespread mosquito-borne virus in the world, and the most common cause of encephalitis in the USA. Surveillance for this medially important mosquito-borne pathogen is an important part of public health practice. Here we present protocols for testing environmental samples such as mosquitoes, nonvertebrate mammals, and birds for this virus, including RT-PCR, virus isolation in cell culture, and antigenic assays, as well as serologic assays for antibody detection.

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Author information

Authors and Affiliations

  1. Wadsworth Center, New York State Department of Health, 5668 State Farm Road, Slingerlands, NY, 12159, USA

    Elizabeth B. Kauffman, Mary A. Franke & Laura D. Kramer

  2. School of Public Health, State University of New York at Albany, Slingerlands, NY, 12159, USA

    Laura D. Kramer

Authors
  1. Elizabeth B. Kauffman
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  2. Mary A. Franke
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  3. Laura D. Kramer
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Corresponding author

Correspondence to Elizabeth B. Kauffman .

Editor information

Editors and Affiliations

  1. Dept. of Path., MicroBio., and Immun., Univ. of South Carolina School of Med., Columbia, South Carolina, USA

    Tonya M. Colpitts

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Kauffman, E.B., Franke, M.A., Kramer, L.D. (2016). Detection Protocols for West Nile Virus in Mosquitoes, Birds, and Nonhuman Mammals. In: Colpitts, T. (eds) West Nile Virus. Methods in Molecular Biology, vol 1435. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3670-0_15

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  • DOI: https://doi.org/10.1007/978-1-4939-3670-0_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3668-7

  • Online ISBN: 978-1-4939-3670-0

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