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Histone Deacetylases pp 15–22Cite as

Detection of Sumo Modification of Endogenous Histone Deacetylase 2 (HDAC2) in Mammalian Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1436))

Abstract

Small ubiquitin-related modifier (SUMO) is an ubiquitin-like protein that is covalently attached to a variety of target proteins and has a significant role in their regulation. HDAC2 is an important epigenetic regulator, promoting the deacetylation of histones and non-histone proteins. HDAC2 has been shown to be modified by SUMO1 at lysine 462. Here we describe how to detect SUMO modification of endogenous HDAC2 in mammalian cells by immunoblotting. Although in this chapter we use this method to detect HDAC2 modification in mammalian cells, this protocol can be used for any cell type or for any protein of interest.

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Acknowledgments

This work was supported by grants from Associazione Italiana per la Ricerca sul Cancro to Susanna Chiocca (AIRC IG5732, AIRC IG12075). SCi was supported by a fellowship from Fondazione Umberto Veronesi (FUV).

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Authors and Affiliations

  1. Department of Experimental Oncology, European Institute of Oncology, IFOM-IEO Campus, 20139, Milan, Italy

    Simona Citro & Susanna Chiocca

Authors
  1. Simona Citro
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  2. Susanna Chiocca
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Corresponding author

Correspondence to Susanna Chiocca .

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Editors and Affiliations

  1. Boston University School of Medicine, Boston, Massachusetts, USA

    Sibaji Sarkar

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Citro, S., Chiocca, S. (2016). Detection of Sumo Modification of Endogenous Histone Deacetylase 2 (HDAC2) in Mammalian Cells. In: Sarkar, S. (eds) Histone Deacetylases. Methods in Molecular Biology, vol 1436. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3667-0_2

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  • DOI: https://doi.org/10.1007/978-1-4939-3667-0_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3665-6

  • Online ISBN: 978-1-4939-3667-0

  • eBook Packages: Springer Protocols

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