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Cloning and Characterization of Sirtuin3 (SIRT3)

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Histone Deacetylases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1436))

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Abstract

Mitochondria play a pivotal role in maintaining cellular homeostasis and regulating longevity. SIRT3 is a mitochondrial sirtuin mediating the deacetylation of various metabolic and antioxidant enzymes, in turn controlling energy metabolism, stress resistance, and the pace of ageing. To study the function of SIRT3, a proteomics-based approach is employed for identifying the protein-binding partners of this enzyme in mitochondria.

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Authors and Affiliations

  1. Department of Pharmacology and Pharmacy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Level 2, Laboratory Block, 21 Sassoon Road, Pokfulam, Hong Kong, China

    Andy W. C. Man, Bo Bai & Yu Wang

Authors
  1. Andy W. C. Man
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  2. Bo Bai
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  3. Yu Wang
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Corresponding author

Correspondence to Yu Wang .

Editor information

Editors and Affiliations

  1. Boston University School of Medicine, Boston, Massachusetts, USA

    Sibaji Sarkar

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© 2016 Springer Science+Business Media New York

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Man, A.W.C., Bai, B., Wang, Y. (2016). Cloning and Characterization of Sirtuin3 (SIRT3). In: Sarkar, S. (eds) Histone Deacetylases. Methods in Molecular Biology, vol 1436. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3667-0_14

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  • DOI: https://doi.org/10.1007/978-1-4939-3667-0_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3665-6

  • Online ISBN: 978-1-4939-3667-0

  • eBook Packages: Springer Protocols

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