Abstract
SIRT2 is a NAD+-dependent deacetylase that belongs to the sirtuin family, which is comprised of seven members (SIRT1-SIRT7) in humans. Furthermore, recent study shows that the Sirt2 gene has three transcript variants in mice. Several diverse proteins have been identified as SIRT2 substrates. SIRT2 activity involves multiple cell processes including growth, differentiation, and energy metabolism. However, little is known of SIRT2’s role in oligodendrocytes or in the myelin sheath, where it is an important component. Here we describe procedures that detail Sirt2 gene cloning, identification, expression, and biological analysis in cultured cells.
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This work was supported by grant from the Canadian Institutes of Health Research and the Saskatchewan Regional Partnership program.
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Ji, S., Doucette, J.R., Nazarali, A.J. (2016). Protocols for Cloning, Expression, and Functional Analysis of Sirtuin2 (SIRT2). In: Sarkar, S. (eds) Histone Deacetylases. Methods in Molecular Biology, vol 1436. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3667-0_13
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DOI: https://doi.org/10.1007/978-1-4939-3667-0_13
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