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Gap Junction Protocols pp 133–144Cite as

Scrape Loading/Dye Transfer Assay

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1437))

Abstract

The scrape loading/dye transfer (SL/DT) technique is a simple functional assay for the simultaneous assessment of gap junctional intercellular communication (GJIC) in a large population of cells. The equipment needs are minimal and are typically met in standard cell biology labs, and SL/DT is the simplest and quickest of all the assays that measure GJIC. This assay has also been adapted for in vivo studies. The SL/DT assay is also conducive to a high-throughput setup with automated fluorescence microscopy imaging and analysis to elucidate more samples in shorter time, and hence can serve a broad range of in vitro pharmacological and toxicological needs.

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Acknowledgements

This work was financially supported by the National Sustainability Programme of the Czech Ministry of Education, Youth and Sports (LO1214) and the RECETOX research infrastructure (LM2015051), and NIEHS grant #R01 ES013268-01A2 to Upham.

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Authors and Affiliations

  1. RECETOX—Research Centre for Toxic Compounds in the Environment, Masaryk University, Kamenice 753/5, Brno, 62500, Czech Republic

    Pavel Babica & Iva Sovadinová

  2. Department of Pediatrics and Human Development, and the Institute for Integrative Toxicology, Michigan State University, East Lansing, MI, USA

    Brad L. Upham

Authors
  1. Pavel Babica
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  2. Iva Sovadinová
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  3. Brad L. Upham
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Corresponding author

Correspondence to Pavel Babica .

Editor information

Editors and Affiliations

  1. Department of in Vitro Toxicology and D, Vrije Universiteit Brussel, Brussels, Belgium

    Mathieu Vinken

  2. BHF Glasgow Cardiovascular Research, University of Glasgow, Glasgow, United Kingdom

    Scott R. Johnstone

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Babica, P., Sovadinová, I., Upham, B.L. (2016). Scrape Loading/Dye Transfer Assay. In: Vinken, M., Johnstone, S. (eds) Gap Junction Protocols. Methods in Molecular Biology, vol 1437. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3664-9_9

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  • DOI: https://doi.org/10.1007/978-1-4939-3664-9_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3662-5

  • Online ISBN: 978-1-4939-3664-9

  • eBook Packages: Springer Protocols

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