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Cholesterol Absorption and Metabolism

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Mouse Models for Drug Discovery

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1438))

Abstract

Inhibitors of cholesterol absorption have been sought for decades as a means to treat and prevent cardiovascular diseases (CVDs) associated with hypercholesterolemia. Ezetimibe is the one clear success story in this regard, and other compounds with similar efficacy continue to be sought. In the last decade, the laboratory mouse, with all its genetic power, has become the premier experimental model for discovering the mechanisms underlying cholesterol absorption and has become a critical tool for preclinical testing of potential pharmaceutical entities. This chapter briefly reviews the history of cholesterol absorption research and the various gene candidates that have come under consideration as drug targets. The most common and versatile method of measuring cholesterol absorption is described in detail along with important considerations when interpreting results, and an alternative method is also presented. In recent years, reverse cholesterol transport (RCT) has become an area of intense new interest for drug discovery since this process is now considered another key to reducing CVD risk. The ultimate measure of RCT is sterol excretion and a detailed description is given for measuring neutral and acidic fecal sterols and interpreting the results.

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Acknowledgments

This work was supported by NIH grants R01HL078900 and R01DK077170-ARRA.

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Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, Center for Lipid and Arteriosclerosis Studies, University of Cincinnati College of Medicine, Metabolic Diseases Institute, 2120 East Galbraith Road, Cincinnati, OH, 45237, USA

    Philip N. Howles Ph.D.

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  1. Philip N. Howles Ph.D.
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Correspondence to Philip N. Howles Ph.D. .

Editor information

Editors and Affiliations

  1. Takeda Pharmaceutical Company, Cambridge, Massachusetts, USA

    Gabriele Proetzel

  2. The Jackson Laboratory, Bar Harbor, Maine, USA

    Michael V. Wiles

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Howles, P.N. (2016). Cholesterol Absorption and Metabolism. In: Proetzel, G., Wiles, M. (eds) Mouse Models for Drug Discovery. Methods in Molecular Biology, vol 1438. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3661-8_11

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  • DOI: https://doi.org/10.1007/978-1-4939-3661-8_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3659-5

  • Online ISBN: 978-1-4939-3661-8

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