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Protocol for Lipid-Mediated Transient Transfection in A549 Epithelial Lung Cell Line

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Molecular Genetics of Asthma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1434))

Abstract

Trials of transfection in eukaryotic cells are essential tools for the study of gene and protein function. They have been used in a wide range of research fields. In this chapter, a method of transient transfection of the A549 cell line, human lung cells of alveolar epithelium, with an expression plasmid is described. In addition, the fundamental characteristics of this experimental procedure are addressed.

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Acknowledgments

This work was supported by grants of the Junta de Castilla y León ref. GRS1047/A/14 and BIO/SA73/15; and by the project “Efecto del Ácido Retinóico en la enfermedad alérgica. Estudio transcripcional y su traslación a la clínica,” PI13/00564, integrated into the “Plan Estatal de I + D + I 2013–2016” and cofunded by the “ISCIII-Subdirección General de Evaluación y Fomento de la investigación” and the European Regional Development Fund (FEDER).

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Authors and Affiliations

  1. Department of Clinical Biochemistry, University Hospital of Salmanca, Paseo de San Vicente 58, Salmanca, 37007, Spain

    Elena Marcos-Vadillo

  2. Salamanca Institute for Biomedical Research (IBSAL), Salamanca, Spain

    Elena Marcos-Vadillo & Asunción García-Sánchez

  3. Department of Biomedical and Diagnostic Sciences, University of Salamanca, Salamanca, Spain

    Asunción García-Sánchez

Authors
  1. Elena Marcos-Vadillo
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  2. Asunción García-Sánchez
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Corresponding author

Correspondence to Elena Marcos-Vadillo .

Editor information

Editors and Affiliations

  1. Department of Clinical Biochemistry, University Hospital of Salamanca, Salamanca, Spain

    María Isidoro García

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Marcos-Vadillo, E., García-Sánchez, A. (2016). Protocol for Lipid-Mediated Transient Transfection in A549 Epithelial Lung Cell Line. In: Isidoro García, M. (eds) Molecular Genetics of Asthma. Methods in Molecular Biology, vol 1434. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3652-6_13

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  • DOI: https://doi.org/10.1007/978-1-4939-3652-6_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3650-2

  • Online ISBN: 978-1-4939-3652-6

  • eBook Packages: Springer Protocols

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