Advertisement

Manipulation of Homologous and Homoeologous Chromosome Recombination in Wheat

  • Adam J. LukaszewskiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1429)

Abstract

Given the sizes of the three genomes in wheat (A, B, and D) and a limited number of chiasmata formed in meiosis, recombination by crossing-over is a very rare event. It is also restricted to very similar homologues; the pairing homoeologous (Ph) system of wheat prevents differentiated chromosomes from pairing and crossing-over. This chapter presents an overview and describes several systems by which the frequency or density of crossing-over can be increased, both in homologues and homoeologues. It also presents the standard system of E.R. Sears for engineering alien chromosome transfers into wheat.

Key words

Triticum aestivum Crossing-over The Ph system Recombination stringency Structural chromosome variants 

References

  1. 1.
    Lukaszewski AJ, Curtis CA (1993) Physical distribution of recombination in B-genome chromosomes of tetraploid wheat. Theor Appl Genet 86:121–127CrossRefPubMedGoogle Scholar
  2. 2.
    Corredor E, Lukaszewski AJ, Pachón P et al (2007) Terminal regions of wheat chromosomes select their pairing partners in meiosis. Genetics 177:609–706CrossRefGoogle Scholar
  3. 3.
    Lukaszewski AJ (2008) Unexpected behavior of an inverted rye chromosome arm in wheat. Chromosoma 117:569–578CrossRefPubMedGoogle Scholar
  4. 4.
    Lukaszewski AJ, Porter DR, Baker CA et al (2001) Attempts to transfer Russian wheat aphid resistance from a rye chromosome in Russian triticales to wheat. Crop Sci 41:1743–1749CrossRefGoogle Scholar
  5. 5.
    Endo TR, Gill BS (1996) The deletion stocks of common wheat. J Hered 87:295–307CrossRefGoogle Scholar
  6. 6.
    Qi LL, Friebe B, Gill BS (2002) A strategy for enhancing recombination in proximal regions of chromosomes. Chromosome Res 10:645–654CrossRefPubMedGoogle Scholar
  7. 7.
    Jones LE, Rybka K, Lukaszewski AJ (2002) The effect of a deficiency and a deletion on recombination in chromosome 1BL in wheat. Theor Appl Genet 104:1204–1208CrossRefPubMedGoogle Scholar
  8. 8.
    Rubnitz J, Subramani S (1984) The minimum amount of homology required for homologous recombination in mammalian cells. Mol Cell Biol 4:2253–2258CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Datta A, Hendrix M, Lipsitch M, Jinks-Robertson S (1997) Dual roles for DNA sequence identity and the mismatch repair system in the regulation of mitotic crossing-over in yeast. Proc Natl Acad Sci U S A 94:9757–9762CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Dvorak J, McGuire P (1981) Nonstructural chromosome differentiation among wheat cultivars with special reference to differentiation of chromosomes in related species. Genetics 97:391–414PubMedPubMedCentralGoogle Scholar
  11. 11.
    Lukaszewski AJ (2000) Manipulation of the 1RS.1BL translocation in wheat by induced homoeologous recombination. Crop Sci 40:216–225CrossRefGoogle Scholar
  12. 12.
    Valenzuela NT, Perrera E, Naranjo T (2013) Identifying crossover-rich regions and their effect on meiotic homologous interactions by partitioning chromosome arms of wheat and rye. Chromosome Res 21:433–445CrossRefPubMedGoogle Scholar
  13. 13.
    Lukaszewski AJ, Kopecky D, Linc G (2012) Inversions of chromosome arms 4AL and 2BS in wheat invert the patterns of chiasma distribution. Chromosoma 121:201–208CrossRefPubMedGoogle Scholar
  14. 14.
    Sears ER (1976) Genetic control of chromosome pairing in wheat. Annu Rev Genet 10:31–51CrossRefPubMedGoogle Scholar
  15. 15.
    Ceoloni C, Donini P (1993) Combining mutations for the two homoeologous pairing suppressor genes Ph1 and Ph2 in common wheat and in hybrids with alien Triticeae. Genome 36:377–386CrossRefPubMedGoogle Scholar
  16. 16.
    Sears ER (1975) An induced homoeologous pairing mutant in wheat. Genetics 80:s74Google Scholar
  17. 17.
    Sears ER (1984) Mutations in wheat that raise the level of meiotic chromosome pairing. In: Gustafson JP (ed) Gene manipulation in plant improvement. 16th Stadler Genetics Symp., Columbia, MO. Plenum Press, New York. p 295–300Google Scholar
  18. 18.
    Gill KS, Gill BS, Endo TR, Mukai Y (1993) Fine mapping of Ph1, a chromosome pairing regulator gene in polyploid wheat. Genetics 134:1231–1236PubMedPubMedCentralGoogle Scholar
  19. 19.
    Sears ER (1954) The aneuploids of common wheat. Mo Agr Exp Sta Res Bull 572:1–58Google Scholar
  20. 20.
    Lukaszewski AJ, Rybka K, Korzun V et al (2004) Genetic and physical mapping of homoeologous recombination points involving wheat chromosome 2B and rye chromosome 2R. Genome 47:36–45CrossRefPubMedGoogle Scholar
  21. 21.
    Naranjo T, Fernandez-Rueda P (1996) Pairing and recombination between individual chromosomes of wheat and rye in hybrids carrying the ph1b mutation. Theor Appl Genet 93:242–248CrossRefPubMedGoogle Scholar
  22. 22.
    Lukaszewski AJ (2006) Cytogenetically engineered rye chromosomes 1R to improve bread-making quality of hexaploid triticale. Crop Sci 46:2183–2194CrossRefGoogle Scholar
  23. 23.
    Lukaszewski AJ (1995) Physical distribution of translocation breakpoints in homoeologous recombinants induced by the absence of the Ph1 gene in wheat and triticale. Theor Appl Genet 90:714–719CrossRefPubMedGoogle Scholar
  24. 24.
    Devos KM, Atkinson MD, Chinoy CN et al (1993) Chromosomal rearrangements in the rye genome relative to wheat. Theor Appl Genet 85:673–680CrossRefPubMedGoogle Scholar
  25. 25.
    Anugrahwati DR, Shepherd KW, Verlin DC et al (2008) Isolation of wheat-rye 1RS recombinants that break the linkage between the stem rust resistance gene SrR and secalins. Genome 51:341–349CrossRefPubMedGoogle Scholar
  26. 26.
    Koebner RMD, Shepherd KW (1986) Controlled introgression to wheat of genes from rye chromosome 1RS by induction of allosyndesis. I. Isolation of recombinants. Theor Appl Genet 73:197–208CrossRefPubMedGoogle Scholar
  27. 27.
    Rogovsky PM, Gudet FLY, Langride P et al (1991) Isolation and characterization of wheat-rye recombinants involving chromosome arm 1DS of wheat. Theor Appl Genet 82:537–544Google Scholar
  28. 28.
    Luo MC, Dubcovsky J, Dvorak J (1996) Recognition of homoeology by the wheat Ph1 locus. Genetics 144:1195–1203PubMedPubMedCentralGoogle Scholar
  29. 29.
    Luo MC, Yang ZL, Kota RS, Dvorak J (2000) Recombination of chromosomes 3Am and 5Am of Triticum monococcum with homoeologous chromosomes 3A and 5A of wheat: distribution of recombination across chromosomes. Genetics 154:1301–1308PubMedPubMedCentralGoogle Scholar
  30. 30.
    Dundas IS, Frappell DE, Crack DM, Fisher JM (2001) Deletion mapping of a nematode resistance gene on rye chromosome 6R in wheat. Crop Sci 41:1771–1778CrossRefGoogle Scholar
  31. 31.
    Dvorak J (1972) Genetic variability in Aegilops speltoides affecting homoeologous pairing in wheat. Can J Genet Cytol 14:317–380CrossRefGoogle Scholar
  32. 32.
    Chen PD, Tsujimoto H, Gill BS (1994) Transfer of Ph I genes promoting homoeologous pairing from Triticum speltoides to common wheat. Theor Appl Genet 88:97–101PubMedGoogle Scholar
  33. 33.
    Sears ER (1981) Transfer of alien genetic material to wheat. In: Evans L, Peacock WJ (eds) Wheat science – today and tomorrow. Cambridge University Press, Cambridge, pp 75–89Google Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of Botany and Plant SciencesUniversity of CaliforniaRiversideUSA

Personalised recommendations