Abstract
Tyramide signal amplification (TSA) fluorescence in situ hybridization (FISH) has been shown as a valuable molecular tool for visualizing specific amplified DNA sequences in chromosome preparations. This chapter describes how to perform TSA-FISH, paying special interest to its two critical steps: probe generation and metaphase plate generation. The potential of physically mapping 12S-globulin sequences by TSA-FISH as a means of identifying homeology among chromosome regions of Avena species was tested and is discussed.
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Acknowledgments
We thank the Ministerio de Ciencia e Innovación of Spain (AGL210-17042) and Universidad de Alcalá (UAH GC2014-002 and UAH CCG2014/EXP-068) for its support of this work.
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Fominaya, A., Loarce, Y., González, J.M., Ferrer, E. (2016). Tyramide Signal Amplification: Fluorescence In Situ Hybridization for Identifying Homoeologous Chromosomes. In: Kianian, S., Kianian, P. (eds) Plant Cytogenetics. Methods in Molecular Biology, vol 1429. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3622-9_4
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DOI: https://doi.org/10.1007/978-1-4939-3622-9_4
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