The Chromosome Microdissection and Microcloning Technique
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Chromosome microdissection followed by microcloning is an efficient tool combining cytogenetics and molecular genetics that can be used for the construction of the high density molecular marker linkage map and fine physical map, the generation of probes for chromosome painting, and the localization and cloning of important genes. Here, we describe a modified technique to microdissect a single chromosome, paint individual chromosomes, and construct single-chromosome DNA libraries.
Key wordsChromosome microdissection and microcloning Degenerated oligonucleotide-primed-PCR Fluorescence in situ hybridization Single-chromosome library
This work was supported by the National Natural Science Foundation of China (31170209) and the Ministry of Science and Technology of China (2011AA100101). We thank Dr. Fangpu Han for kindly providing repetitive sequence pSc119.2 and the technique advice on FISH.
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