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The Chromosome Microdissection and Microcloning Technique

  • Ying-Xin Zhang
  • Chuan-Liang Deng
  • Zan-Min HuEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1429)

Abstract

Chromosome microdissection followed by microcloning is an efficient tool combining cytogenetics and molecular genetics that can be used for the construction of the high density molecular marker linkage map and fine physical map, the generation of probes for chromosome painting, and the localization and cloning of important genes. Here, we describe a modified technique to microdissect a single chromosome, paint individual chromosomes, and construct single-chromosome DNA libraries.

Key words

Chromosome microdissection and microcloning Degenerated oligonucleotide-primed-PCR Fluorescence in situ hybridization Single-chromosome library 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (31170209) and the Ministry of Science and Technology of China (2011AA100101). We thank Dr. Fangpu Han for kindly providing repetitive sequence pSc119.2 and the technique advice on FISH.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijingChina
  2. 2.Graduate University of Chinese Academy of SciencesBeijingP. R. China
  3. 3.Henan Normal UniversityXinxiangChina

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