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Plant Cytogenetics pp 151–160Cite as

The Chromosome Microdissection and Microcloning Technique

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1429))

Abstract

Chromosome microdissection followed by microcloning is an efficient tool combining cytogenetics and molecular genetics that can be used for the construction of the high density molecular marker linkage map and fine physical map, the generation of probes for chromosome painting, and the localization and cloning of important genes. Here, we describe a modified technique to microdissect a single chromosome, paint individual chromosomes, and construct single-chromosome DNA libraries.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31170209) and the Ministry of Science and Technology of China (2011AA100101). We thank Dr. Fangpu Han for kindly providing repetitive sequence pSc119.2 and the technique advice on FISH .

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Author notes

    Authors and Affiliations

    1. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing, 100101, China

      Ying-Xin Zhang & Zan-Min Hu

    2. Graduate University of Chinese Academy of Sciences, Beijing, 100049, P. R. China

      Ying-Xin Zhang

    3. Henan Normal University, Xinxiang, 453007, China

      Chuan-Liang Deng

    Authors
    1. Ying-Xin Zhang
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    2. Chuan-Liang Deng
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    3. Zan-Min Hu
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    Corresponding author

    Correspondence to Zan-Min Hu .

    Editor information

    Editors and Affiliations

    1. University of Minnesota, St. Paul, Minnesota, USA

      Shahryar F. Kianian

    2. University of Minnesota, St. Paul, Minnesota, USA

      Penny M. Avoles Kianian

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    Zhang, YX., Deng, CL., Hu, ZM. (2016). The Chromosome Microdissection and Microcloning Technique. In: Kianian, S., Kianian, P. (eds) Plant Cytogenetics. Methods in Molecular Biology, vol 1429. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3622-9_12

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    • DOI: https://doi.org/10.1007/978-1-4939-3622-9_12

    • Published:

    • Publisher Name: Humana Press, New York, NY

    • Print ISBN: 978-1-4939-3620-5

    • Online ISBN: 978-1-4939-3622-9

    • eBook Packages: Springer Protocols

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