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Plant Cytogenetics pp 119–134Cite as

Flow Sorting Plant Chromosomes

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1429))

Abstract

Nuclear genomes of many important plant species are tremendously complicated to map and sequence. The ability to isolate single chromosomes, which represent small units of nuclear genome, is priceless in many areas of plant research including cytogenetics, genomics, and proteomics. Flow cytometry is the only technique which can provide large quantities of pure chromosome fractions suitable for downstream applications including physical mapping, preparation of chromosome-specific BAC libraries, sequencing, and optical mapping. Here, we describe step-by-step procedure of preparation of liquid suspensions of intact mitotic metaphase chromosomes and their flow cytometric analysis and sorting.

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Acknowledgment

This work was supported by grant LO1204 from the National Program of Sustainability I. We also thank Ms. Zdeňka Dubská and Ms. Romana Šperková for excellent technical assistance.

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Authors and Affiliations

  1. Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Šlechtitelů 31, 783 71, Olomouc, Czech Republic

    Jan Vrána, Petr Cápal, Jarmila Číhalíková, Marie Kubaláková & Jaroslav Doležel

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  1. Jan Vrána
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  2. Petr Cápal
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  3. Jarmila Číhalíková
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  4. Marie Kubaláková
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  5. Jaroslav Doležel
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Correspondence to Jan Vrána .

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Editors and Affiliations

  1. University of Minnesota, St. Paul, Minnesota, USA

    Shahryar F. Kianian

  2. University of Minnesota, St. Paul, Minnesota, USA

    Penny M. Avoles Kianian

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Vrána, J., Cápal, P., Číhalíková, J., Kubaláková, M., Doležel, J. (2016). Flow Sorting Plant Chromosomes. In: Kianian, S., Kianian, P. (eds) Plant Cytogenetics. Methods in Molecular Biology, vol 1429. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3622-9_10

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  • DOI: https://doi.org/10.1007/978-1-4939-3622-9_10

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